coli, a flavin reductase (Fre) that regenerates FADH 2 for the halogenase reaction was used as an N-terminal soluble tag of SttH. Since tryptophan halogenases are expressed in highly insoluble forms in E. Here, we introduce an effective 6BrIG production strategy in Escherichia coli using tryptophan 6-halogenase SttH, tryptophanase TnaA and flavin-containing monooxygenase MaFMO. However, its synthesis remains limited due to uncharacterized biosynthetic pathways and the difficulty of regiospecific bromination. Tyrian purple, mainly composed of 6,6'-dibromoindigo (6BrIG), is an ancient dye extracted from sea snails and was recently demonstrated as a biocompatible semiconductor material.
0 kommentar(er)
